Snyder L (1986) High performance liquid chromatography–advances and perspectives. Snyder LR, Dolan JW (2007) High-performance gradient elution-the practical application of linear-solvent-strength model. Gomis DB, Nunez NS, Garcia EA, Abrodo PA, Alvarez MDG (2006) J Liq Chromatogr Relat Technol 29:1861–1875 Gritti F, Leonardis I, Abia J, Guiochon G (2010) J Chromatogr A 1217:3819–3843 Gritti F, Leonardis I, Shock D, Stevenson P, Shalliker A, Guiochon G (2010) J Chromatogr A 1217:1589–1603 S:55–59Ībrahim A, Al-Sayah M, Skrdla P, Bereznitski Y, Chen Y, Wu N (2010) J Pharm Biomed Anal 51:131–137 Koerner P, Mathews T (2010) LC GC North Am. ![]() Way WK, Brandes H (2008) LC GC North Am Suppl S:64–64ĭeStefano JJ, Langlois TJ, Kirkland JJ (2008) J Chromatogr Sci 46:254–260 Klein EJ, Rivera SL (2000) J Liq Chromatogr Relat Technol 23:2097–2121 Pragst F, Herzler M, Erxleben BT (2004) Clin Chem Lab Med 42:1325–1340 Stroh JG, Petucci CJ, Brecker SJ, Nogle LM (2008) J Sep Sci 31:3698–3703 Moreover, application of Kinetex C18 column for the analysis of macrolides under alkaline conditions was limited with respect to its decreasing performance with growing number of injections on the column. On the contrary, the maximal experimental peak capacities for analysis of macrolides on Acquity BEH C18 column was higher (46.7) than that on Kinetex C18 column (36.9). ![]() This indicated that Kinetex C18 was a suitable alternative to Acquity BEH C18 column for the analysis of tetracyclines under acidic conditions. These core-shell particles also have exceptionally consistent shape and size, which helps to improve separation efficiency by reducing the variability in analyte movement between particles. The maximal experimental peak capacities for analysis of tetracyclines were 51.8 (Acquity BEH C18 column) and 48.4 (Kinetex C18 column). A 2.6 m Kinetex core-shell particle has a non-porous core approximately 1.9 m in diameter and an outer porous layer of 0.35 m. ![]() The columns were compared by evaluation of their experimental peak capacity and its dependence on linear velocity and gradient slope. The performance of Kinetex C18 column (2.1 × 50 mm) and Acquity BEH C18 column (2.1 × 50 mm) for gradient separation of tetracyclines under acidic conditions (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) and macrolides under alkaline conditions (tylosin, clarithromycin, roxithromycin, and carbomycin) was studied. Kinetex Core-Shell Columns Earn the Gold Seal of Quality Read More. Unlike Acquity BEH C18 column, Kinetex C18 column exhibited lower column backpressure making this column compatible to conventional LC systems. The recently introduced Kinetex C18 column packed with core-shell 2.6 μm particles is declared to provide similar efficiency and short analysis as Acquity BEH C18 column with 1.7 μm porous particles.
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